The road starts in the United Kingdom, however at no point in the UK, like all European routes, is it signed by the name E05. Also, the route does not follow one particular UK road but follows its own route.
On the M8 motorway the route goes through Glasgow before diverging to the M74, with the remainder of the M8 (which forms the UK section of the E16 route
The M74 becomes the A74(M) at Abington and continues south passing through Gretna where the E18 route (the A75) joins, before turning into the A74 at the border for a short distance, subsequently turning into the M6 through England.